Comparative study on density gradients and swim-up preparation techniques utilizing neat and cryopreserved spermatozoa
نویسندگان
چکیده
منابع مشابه
Comparative study on density gradients and swim-up preparation techniques utilizing neat and cryopreserved spermatozoa.
AIM To 1) compare post-wash and post-thaw parameters of sperm processed with PureSperm density gradient technique and swim-up method; and 2) test the efficacy of two commonly available density gradient media PureSperm and ISolate. METHODS This prospective study used semen specimens from 22 patients. Specimens from nine patients were processed by both PureSperm density gradient and swim-up met...
متن کاملComparative proteomic analysis of spermatozoa isolated by swim-up or density gradient centrifugation
BACKGROUND Reports about the morphologic and functional characteristics of spermatozoa prepared by density gradient centrifugation (DC) or swim-up (SU) have produced discordant results. We have performed a proteomic comparison of cells prepared by DC and SU providing a molecular insight into the differences between these two methods of sperm cell isolation. METHODS Protein maps were obtained ...
متن کاملImprovement in motion characteristics and acrosome status in cryopreserved human spermatozoa by swim-up processing before freezing.
The purpose of this study was to examine if selecting a sperm population with improved motion characteristics before freezing reduces the deleterious effects of cryopreservation. Semen specimens from 15 normal donors were divided into two equal aliquots. The first aliquot received no treatment (control), and the second was processed by swim-up from a washed sperm preparation to select a sperm p...
متن کاملmIgratIon-based technIques swim-up
This chapter will discuss the various sperm preparation and selection techniques used to process sperm for use with assisted reproductive techniques: swim-down, swim-up, migrationsedimentation, density gradient centrifugation, magnetic activated cell sorting, and glass wool filtration. It will also explain the procedures used to prepare viscous semen samples as well as when to obtain and prepar...
متن کاملCan DNA fragmentation of neat or swim-up spermatozoa be used to predict pregnancy following ICSI of fertile oocyte donors?
This study compared the potential of assessing sperm DNA fragmentation (SDF) from neat semen and the subsequent swim-up (SU) procedure to predict pregnancy when conducting ICSI of fertile donor oocytes. Infertile females (n=81) were transferred embryos resulting from intracytoplasmic sperm injection (ICSI) of their partner's spermatozoa and proven donor oocytes. This model normalized the impact...
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ژورنال
عنوان ژورنال: Asian Journal of Andrology
سال: 2005
ISSN: 1008-682X,1745-7262
DOI: 10.1111/j.1745-7262.2005.00008.x